Animal Influenza Virus by Erica Spackman

Author:Erica Spackman
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

15.Enumerate spots on ELISPOT reader (Fig. 2).

Fig. 2Total IgG ELISPOT from splenic B cells (see Subheading 3.5.1). Four-week-old SPF chickens were vaccinated with H5N9 avian influenza virus. Spleen cells were harvested through mesh screen as above. IgG positive cells plotted by total number of cells per well (a). Captured image of positive spots in wells containing 5 × 105, 2.5 × 105, and 1.25 × 105 total cells (b). Approximately 200 IgG ASC B-cells were detected in 5 × 105 total splenocytes

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